Evaluation of Lacc134 Oxidoreductase of Ganoderma multistipitatum in Detoxification of Dye Wastewater under Different Nutritional Conditions

In the present study, we investigated effects of different carbon sources (glucose, sucrose, and maltose) on laccase production from mycelium Ganoderma multistipitatum grown malt extract agar plates. The preliminary screening test was performed guaiacol plate, where a maroon brown zone formed after oxidation. A few pure mycelial discs species were transferred into submerged fermentation nutrient broth. medium at 20 g glucose revealed highest activities (2300 U/L) than other sources. interesting results also shown by inorganic NaNO3 in maximum (7800 ± 1.1 U/L). organic nitrogen inducer, namely yeast extract, exhibited 5834 U/L activity potential source secretion. concluded that C N inducers enhanced production. This process is eco-friendly effective removal dye water. Laccase cultural broth partially purified SDS-PAGE for molecular weight determination, while Native-PAGE confirmed band staining with guaiacol. Km Vmax values Lacc134 1.658 mm 2.452 mM min−1, respectively. this study effectively removed Remazol Brilliant Blue R (RBBR) (extensively used textile industries wastewater). For capacity, 2.0 mg, 4.0 5.0 6.0 mg used, which most (85% 88%) concentration 1st 2nd h interval treatment, Total (TOC) quantity percentage first- second-hour time 62% 89%, respectively, 30 glucose. According to experimental finding breakdown products catalyzed are less hazardous due lower itself.